Wasps winger Paolo Odogwu and Bristol scrum-half Harry Randall have been given their first call-ups to the England squad for the upcoming Six Nations.
The uncapped duo have been included in a 28-man group for the championship, which starts at the beginning of next month.
Head coach Eddie Jones has also named a 'shadow squad' of twelve players who will be part of the same testing protocols as the tournament squad. Mako Vunipola will also join them to continue his rehabilitation from injury.
The smaller-than-usual main panel of 28 is to reduce the risk of infection.
Jones is currently isolating after assistant Matt Proudfoot tested positive for coronavirus.
England's campaign begins with the Calcutta Cup match with Scotland at Twickenham on February 6.
"This has been a really tough squad to pick," Jones said, "And I know there are a lot of disappointed players who haven’t been selected. We’re really excited by the players we have got, it’s a very vibrant squad and are looking forward to getting started next week.
“We’re very grateful to the Premiership Rugby, the clubs and RPA for allowing us to have 28 players throughout the tournament, we’re very happy with the number and it’s testament to the growing relationships between all parties.
“Ultimately our goal is to win the Six Nations and to do that we’ll need to work hard and prepare well each day and take it as it comes.”
England Six Nations squad:
Elliot Daly (Saracens, 47 caps)
Owen Farrell (Saracens, 88 caps)
George Ford (Leicester Tigers, 72 caps)
Ollie Lawrence (Worcester Warriors, 3 caps)
Max Malins (Bristol Bears, 3 caps)
Jonny May (Gloucester Rugby, 61 caps)
Paolo Odogwu (Wasps, uncapped)
Harry Randall (Bristol Bears, uncapped)
Dan Robson (Wasps, 7 caps)
Henry Slade (Exeter Chiefs, 34 caps)
Anthony Watson (Bath Rugby, 46 caps)
Ben Youngs (Leicester Tigers, 104 caps)
Luke Cowan-Dickie (Exeter Chiefs, 26 caps)
Tom Curry (Sale Sharks, 28 caps)
Ben Earl (Bristol Bears, 8 caps)
Ellis Genge (Leicester Tigers, 23 caps)
Jamie George (Saracens, 54 caps)
Jonny Hill (Exeter Chiefs, 3 caps)
Maro Itoje (Saracens, 43 caps)
Joe Launchbury (Wasps, 69 caps)
Courtney Lawes (Northampton Saints, 85 caps)
Joe Marler (Harlequins, 72 caps)
Beno Obano (Bath Rugby, uncapped)
Will Stuart (Bath Rugby, 8 caps)
Sam Underhill (Bath Rugby, 22 caps)
Billy Vunipola (Saracens, 56 caps)
Harry Williams (Exeter Chiefs, 18 caps)
Mark Wilson (Newcastle Falcons, 19 caps)
Charlie Atkinson (Wasps, uncapped)
Ali Crossdale (Saracens, uncapped)
Tom Dunn (Bath Rugby, 3 caps)
Charlie Ewels (Bath Rugby, 17 caps)
George Furbank (Northampton Saints, 3 caps)
Joe Heyes (Leicester Tigers, uncapped)
Jonathan Joseph (Bath Rugby, 54 caps)
Joe Marchant (Harlequins, 4 caps)
George Martin (Leicester Tigers, uncapped)
Alex Mitchell (Northampton Saints, uncapped)
Jacob Umaga (Wasps, uncapped)
Jack Willis (Wasps, 2 caps)