Team Ireland has now won six medals at the Paralympics, with Katie-George Dunlevy and Eve McCrystal claiming gold in the time trial.
The cyclists produced a commanding performance on the road in Tokyo, finishing almost a minute in front of Great Britain in second place.
They came home in a time of 47 minutes 32 point zero seven seconds.
It's Dunlevy and McCrystal's second medal of the games - they took silver in the Velodrome last Saturday.
They have also matched their performance at the 2016 Paralympics in Rio when they brought home gold and silver medals.
Earlier, hand cyclist Gary O'Reilly from Laois claimed bronze in the H5 Time Trial, winning a medal in his first Paralympics.
He clocked a time of 39 minutes 36 point four six seconds on the road.
Ronan Grimes ended in sixth place in the C4 Time Trial Final.
Richael Timothy finished 14th in the C1-3 Time Trial.
And Orla Comerford was 4th in her heat in the T13 100 metres, a result which was not enough to reach the final.